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Cytokines play an aetiopathogenetic role in fibromyalgia: a hypothesis and pilot study.

Abstract

OBJECTIVE

To measure soluble factors having a possible role in fibromyalgia (FM) and compare the profiles of patients with recent onset of the syndrome with patients with chronic FM.

METHODS

The production of cytokines, cytokine-related molecules, and a CXC chemokine, interleukin (IL)-8, was examined. Fifty-six patients with FM (23 with <2 yr and 33 with >2 yr of symptoms) were compared with age- and sex-matched healthy controls. Cytokines and cytokine-related molecules were measured in sera and in supernatants of peripheral blood mononuclear cells (PBMC) that were incubated with and without lectins and phorbol myristate acetate (PMA).

RESULTS

No differences between FMS and controls were found by measuring IL-1beta, IL-2, IL-10, serum IL-2 receptor (sIL-2R), interferon gamma (IFN-gamma), and tumour necrosis factor alpha (TNF-alpha). Levels of IL-1R antibody (IL-1Ra) and IL-8 were significantly higher in sera, and IL-1Ra and IL-6 were significantly higher in stimulated and unstimulated FM PBMC compared with controls. Serum IL-6 levels were comparable to those in controls, but were elevated in supernatants of in vitro-activated PBMC derived from patients with >2 yr of symptoms. In the presence of PMA, there were additional increases in IL-1Ra, IL-8 and IL-6 over control values.

CONCLUSIONS

In patients with FM we found increases over time in serum levels and/or PBMC-stimulated activity of soluble factors whose release is stimulated by substance P. Because IL-8 promotes sympathetic pain and IL-6 induces hyperalgesia, fatigue and depression, it is hypothesized that they may play a role in modulating FM symptoms.

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  • Authors+Show Affiliations

    ,

    Department of Medicine/Division of Rheumatology, Cedars-Sinai Medical Center/UCLA School of Medicine, Los Angeles, CA, USA.

    , , , ,

    Source

    Rheumatology (Oxford, England) 40:7 2001 Jul pg 743-9

    MeSH

    Cytokines
    Enzyme-Linked Immunosorbent Assay
    Female
    Fibromyalgia
    Humans
    Lipopolysaccharides
    Lymphocyte Activation
    Male
    Middle Aged
    Monocytes
    Phytohemagglutinins
    Pilot Projects
    Surveys and Questionnaires
    Tetradecanoylphorbol Acetate

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    11477278

    Citation

    Wallace, D J., et al. "Cytokines Play an Aetiopathogenetic Role in Fibromyalgia: a Hypothesis and Pilot Study." Rheumatology (Oxford, England), vol. 40, no. 7, 2001, pp. 743-9.
    Wallace DJ, Linker-Israeli M, Hallegua D, et al. Cytokines play an aetiopathogenetic role in fibromyalgia: a hypothesis and pilot study. Rheumatology (Oxford). 2001;40(7):743-9.
    Wallace, D. J., Linker-Israeli, M., Hallegua, D., Silverman, S., Silver, D., & Weisman, M. H. (2001). Cytokines play an aetiopathogenetic role in fibromyalgia: a hypothesis and pilot study. Rheumatology (Oxford, England), 40(7), pp. 743-9.
    Wallace DJ, et al. Cytokines Play an Aetiopathogenetic Role in Fibromyalgia: a Hypothesis and Pilot Study. Rheumatology (Oxford). 2001;40(7):743-9. PubMed PMID: 11477278.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Cytokines play an aetiopathogenetic role in fibromyalgia: a hypothesis and pilot study. AU - Wallace,D J, AU - Linker-Israeli,M, AU - Hallegua,D, AU - Silverman,S, AU - Silver,D, AU - Weisman,M H, PY - 2001/7/31/pubmed PY - 2001/8/24/medline PY - 2001/7/31/entrez SP - 743 EP - 9 JF - Rheumatology (Oxford, England) JO - Rheumatology (Oxford) VL - 40 IS - 7 N2 - OBJECTIVE: To measure soluble factors having a possible role in fibromyalgia (FM) and compare the profiles of patients with recent onset of the syndrome with patients with chronic FM. METHODS: The production of cytokines, cytokine-related molecules, and a CXC chemokine, interleukin (IL)-8, was examined. Fifty-six patients with FM (23 with <2 yr and 33 with >2 yr of symptoms) were compared with age- and sex-matched healthy controls. Cytokines and cytokine-related molecules were measured in sera and in supernatants of peripheral blood mononuclear cells (PBMC) that were incubated with and without lectins and phorbol myristate acetate (PMA). RESULTS: No differences between FMS and controls were found by measuring IL-1beta, IL-2, IL-10, serum IL-2 receptor (sIL-2R), interferon gamma (IFN-gamma), and tumour necrosis factor alpha (TNF-alpha). Levels of IL-1R antibody (IL-1Ra) and IL-8 were significantly higher in sera, and IL-1Ra and IL-6 were significantly higher in stimulated and unstimulated FM PBMC compared with controls. Serum IL-6 levels were comparable to those in controls, but were elevated in supernatants of in vitro-activated PBMC derived from patients with >2 yr of symptoms. In the presence of PMA, there were additional increases in IL-1Ra, IL-8 and IL-6 over control values. CONCLUSIONS: In patients with FM we found increases over time in serum levels and/or PBMC-stimulated activity of soluble factors whose release is stimulated by substance P. Because IL-8 promotes sympathetic pain and IL-6 induces hyperalgesia, fatigue and depression, it is hypothesized that they may play a role in modulating FM symptoms. SN - 1462-0324 UR - https://www.unboundmedicine.com/medline/citation/11477278/full_citation L2 - https://academic.oup.com/rheumatology/article-lookup/doi/10.1093/rheumatology/40.7.743 DB - PRIME DP - Unbound Medicine ER -