Tags

Type your tag names separated by a space and hit enter

HLA-DQ typing in the diagnosis of celiac disease.
Am J Gastroenterol 2002; 97(3):695-9AJ

Abstract

OBJECTIVE

More than 95% of celiac patients share the major histocompatibility complex II class human leukocyte antigen (HLA) DQ2 or DQ8 haplotype; patients negative for both types are unlikely to suffer from celiac disease. Our aim was to investigate whether HLA-DQ2 and -DQ8 typing is helpful when diagnosis is uncertain because of the absence of unequivocal small bowel villous atrophy.

METHODS

HLA-DQ2 and -DQ8 typing was carried out in 59 patients evincing nondiagnostic small bowel mucosal lesion or positive celiac serology, and in 17 patients maintaining a gluten-free diet without biopsy-proven celiac disease. HLA findings were compared to small bowel mucosal morphology; intraepithelial lymphocytes; and serum endomysial (EmA), reticulin, tissue transglutaminase (anti-tTG) and gliadin antibodies.

RESULTS

Of the 59 patients evincing only minor small bowel mucosal changes or positive celiac disease serology, 22 (37%) were negative for DQ2 and DQ8. All EmA-positive patients had celiac-type HLA, but antireticulin antibody, anti-tTg, and antigliadin antibody were also present in HLA-DQ2- and -DQ8-negative individuals. Eleven of 17 patients (65%) observing a gluten-free diet before small bowel biopsy did not share celiac-type HLA. None of the 17 had apparent villous atrophy. Serum EmA and anti-tTG were negative in all. HLA-DQ typing is less expensive than follow-up biopsy in the exclusion of celiac disease.

CONCLUSIONS

HLA-DQ2 and -DQ8 determination is useful in exclusion, probably lifelong, of celiac disease in individuals with an equivocal small bowel histological finding. The low specificity of this test must, however, be borne in mind.

Authors+Show Affiliations

Department of Internal Medicine, Tampere University Hospital, Finland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11922565

Citation

Kaukinen, Katri, et al. "HLA-DQ Typing in the Diagnosis of Celiac Disease." The American Journal of Gastroenterology, vol. 97, no. 3, 2002, pp. 695-9.
Kaukinen K, Partanen J, Mäki M, et al. HLA-DQ typing in the diagnosis of celiac disease. Am J Gastroenterol. 2002;97(3):695-9.
Kaukinen, K., Partanen, J., Mäki, M., & Collin, P. (2002). HLA-DQ typing in the diagnosis of celiac disease. The American Journal of Gastroenterology, 97(3), pp. 695-9.
Kaukinen K, et al. HLA-DQ Typing in the Diagnosis of Celiac Disease. Am J Gastroenterol. 2002;97(3):695-9. PubMed PMID: 11922565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA-DQ typing in the diagnosis of celiac disease. AU - Kaukinen,Katri, AU - Partanen,Jukka, AU - Mäki,Markku, AU - Collin,Pekka, PY - 2002/4/2/pubmed PY - 2002/4/20/medline PY - 2002/4/2/entrez SP - 695 EP - 9 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 97 IS - 3 N2 - OBJECTIVE: More than 95% of celiac patients share the major histocompatibility complex II class human leukocyte antigen (HLA) DQ2 or DQ8 haplotype; patients negative for both types are unlikely to suffer from celiac disease. Our aim was to investigate whether HLA-DQ2 and -DQ8 typing is helpful when diagnosis is uncertain because of the absence of unequivocal small bowel villous atrophy. METHODS: HLA-DQ2 and -DQ8 typing was carried out in 59 patients evincing nondiagnostic small bowel mucosal lesion or positive celiac serology, and in 17 patients maintaining a gluten-free diet without biopsy-proven celiac disease. HLA findings were compared to small bowel mucosal morphology; intraepithelial lymphocytes; and serum endomysial (EmA), reticulin, tissue transglutaminase (anti-tTG) and gliadin antibodies. RESULTS: Of the 59 patients evincing only minor small bowel mucosal changes or positive celiac disease serology, 22 (37%) were negative for DQ2 and DQ8. All EmA-positive patients had celiac-type HLA, but antireticulin antibody, anti-tTg, and antigliadin antibody were also present in HLA-DQ2- and -DQ8-negative individuals. Eleven of 17 patients (65%) observing a gluten-free diet before small bowel biopsy did not share celiac-type HLA. None of the 17 had apparent villous atrophy. Serum EmA and anti-tTG were negative in all. HLA-DQ typing is less expensive than follow-up biopsy in the exclusion of celiac disease. CONCLUSIONS: HLA-DQ2 and -DQ8 determination is useful in exclusion, probably lifelong, of celiac disease in individuals with an equivocal small bowel histological finding. The low specificity of this test must, however, be borne in mind. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/11922565/full_citation L2 - http://Insights.ovid.com/pubmed?pmid=11922565 DB - PRIME DP - Unbound Medicine ER -