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Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins.
Eur J Clin Nutr 2004; 58(2):317-25EJ

Abstract

OBJECTIVE

We investigated whether ingestion of polyphenols from fruit juices or a fruit-vegetable-concentrate affects lymphocyte proliferation and apoptosis in human immunodeficiency virus (HIV)-seropositive (HIV(+)) and HIV-seronegative (HIV(-)) subjects.

DESIGN

Randomized, prospective pilot intervention study.

SETTING

University of Bonn, Department of General Internal Medicine.

SUBJECTS

A total of 23 HIV(+) subjects from the HIV outpatient clinic, 18 HIV(-) controls.

INTERVENTIONS

Subjects ingested either 1 l of fruit juice or 30 ml of fruit-vegetable-concentrate daily for 16 weeks in addition to their regular diet. Lymphocyte proliferation and apoptosis were investigated in peripheral blood mononuclear cells at baseline, during 16-weeks of intervention, and after a 6-week washout. Proliferation was assessed by (3)H-thymidine incorporation and apoptosis by nuclear content as measured by flow cytometry.

RESULTS

Supplementation of fruit juices increased phytohemagglutinin-induced lymphocyte proliferation (mitotic index) in HIV(+) patients from 18+/-16 to 40+/-34 (P=0.004) and in healthy controls from 27+/-16 to 51+/-21 (P=0.016). Apoptosis was not affected in HIV(+) patients, but rose in healthy controls from 9+/-10 to 34+/-11 (apoptotic index; P=0.001). Intervention with concentrate did not significantly alter proliferation and apoptosis in HIV(+) and HIV(-) subjects.

CONCLUSIONS

Even though apoptosis did not change in HIV(+) subjects, ingestion of polyphenol-rich fruit juices might be favorable to HIV(+) patients due to enhanced proliferation, which could restore disturbances in T-cell homeostasis. In healthy controls, increased lymphocyte proliferation during juice consumption was counterbalanced by increased apoptosis.

Authors+Show Affiliations

School of Medicine, Department of Hemostasis and Transfusion Medicine, University of Duesseldorf, Duesseldorf, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14749753

Citation

Winkler, P, et al. "Lymphocyte Proliferation and Apoptosis in HIV-seropositive and Healthy Subjects During Long-term Ingestion of Fruit Juices or a Fruit-vegetable-concentrate Rich in Polyphenols and Antioxidant Vitamins." European Journal of Clinical Nutrition, vol. 58, no. 2, 2004, pp. 317-25.
Winkler P, Ellinger S, Boetzer AM, et al. Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins. Eur J Clin Nutr. 2004;58(2):317-25.
Winkler, P., Ellinger, S., Boetzer, A. M., Arendt, B. M., Berthold, H. K., Rockstroh, J. K., ... Goerlich, R. (2004). Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins. European Journal of Clinical Nutrition, 58(2), pp. 317-25.
Winkler P, et al. Lymphocyte Proliferation and Apoptosis in HIV-seropositive and Healthy Subjects During Long-term Ingestion of Fruit Juices or a Fruit-vegetable-concentrate Rich in Polyphenols and Antioxidant Vitamins. Eur J Clin Nutr. 2004;58(2):317-25. PubMed PMID: 14749753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lymphocyte proliferation and apoptosis in HIV-seropositive and healthy subjects during long-term ingestion of fruit juices or a fruit-vegetable-concentrate rich in polyphenols and antioxidant vitamins. AU - Winkler,P, AU - Ellinger,S, AU - Boetzer,A M, AU - Arendt,B M, AU - Berthold,H K, AU - Rockstroh,J K, AU - Spengler,U, AU - Goerlich,R, PY - 2004/1/30/pubmed PY - 2004/4/20/medline PY - 2004/1/30/entrez SP - 317 EP - 25 JF - European journal of clinical nutrition JO - Eur J Clin Nutr VL - 58 IS - 2 N2 - OBJECTIVE: We investigated whether ingestion of polyphenols from fruit juices or a fruit-vegetable-concentrate affects lymphocyte proliferation and apoptosis in human immunodeficiency virus (HIV)-seropositive (HIV(+)) and HIV-seronegative (HIV(-)) subjects. DESIGN: Randomized, prospective pilot intervention study. SETTING: University of Bonn, Department of General Internal Medicine. SUBJECTS: A total of 23 HIV(+) subjects from the HIV outpatient clinic, 18 HIV(-) controls. INTERVENTIONS: Subjects ingested either 1 l of fruit juice or 30 ml of fruit-vegetable-concentrate daily for 16 weeks in addition to their regular diet. Lymphocyte proliferation and apoptosis were investigated in peripheral blood mononuclear cells at baseline, during 16-weeks of intervention, and after a 6-week washout. Proliferation was assessed by (3)H-thymidine incorporation and apoptosis by nuclear content as measured by flow cytometry. RESULTS: Supplementation of fruit juices increased phytohemagglutinin-induced lymphocyte proliferation (mitotic index) in HIV(+) patients from 18+/-16 to 40+/-34 (P=0.004) and in healthy controls from 27+/-16 to 51+/-21 (P=0.016). Apoptosis was not affected in HIV(+) patients, but rose in healthy controls from 9+/-10 to 34+/-11 (apoptotic index; P=0.001). Intervention with concentrate did not significantly alter proliferation and apoptosis in HIV(+) and HIV(-) subjects. CONCLUSIONS: Even though apoptosis did not change in HIV(+) subjects, ingestion of polyphenol-rich fruit juices might be favorable to HIV(+) patients due to enhanced proliferation, which could restore disturbances in T-cell homeostasis. In healthy controls, increased lymphocyte proliferation during juice consumption was counterbalanced by increased apoptosis. SN - 0954-3007 UR - https://www.unboundmedicine.com/medline/citation/14749753/full_citation L2 - http://dx.doi.org/10.1038/sj.ejcn.1601784 DB - PRIME DP - Unbound Medicine ER -