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Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma.

Abstract

Multidrug-resistant enteric bacteria were isolated from turkey, cattle, and chicken farms and retail meat products in Oklahoma. Among the isolated species, multidrug-resistant Klebsiella pneumoniae was prevalently isolated from most of the collected samples. Therefore, a total of 132 isolates of K. pneumoniae were characterized to understand their potential roles in the dissemination of antibiotic-resistance genes in the food chains. Multidrug-resistant K. pneumoniae was most frequently recovered from a turkey farm and ground turkey products among the tested samples. All isolates were resistant to ampicillin, tetracycline, streptomycin, gentamycin, and kanamycin. Class 1 integrons located in plasmids were identified as a common carrier of the aadA1 gene, encoding resistance to streptomycin and spectinomycin. Production of beta-lactamase in the K. pneumoniae isolates played a major role in the resistance to beta-lactam agents. Most isolates (96%) possessed bla(SHV1). Five strains were able to express both SHV-11 (pI 6.2) and TEM-1 (pI 5.2) beta-lactamase. Transfer of these antibiotic-resistance genes to Escherichia coli was demonstrated by transconjugation. The bacterial genomic DNA restriction patterns by pulsed-field gel electrophoresis showed that the same clones of multidrug-resistant K. pneumoniae remained in feathers, feed, feces, and drinking water in turkey environments, indicating the possible dissemination of antibiotic-resistance genes in the ecosystem and cross-contamination of antibiotic-resistant bacteria during processing and distribution of products.

Links

Authors+Show Affiliations

,

Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. kimshin@okstate.edu

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Source

Journal of food protection 68:10 2005 Oct pg 2022-9

MeSH

Animal Husbandry
Animals
Anti-Bacterial Agents
Base Sequence
Cattle
Chickens
Colony Count, Microbial
Conjugation, Genetic
Consumer Product Safety
DNA, Bacterial
Drug Resistance, Multiple, Bacterial
Food Contamination
Food Handling
Klebsiella pneumoniae
Meat
Meat Products
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation
Oklahoma
Plasmids
Polymerase Chain Reaction
Turkeys
beta-Lactam Resistance
beta-Lactamases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16245702

Citation

Kim, Shin-Hee, et al. "Multidrug-resistant Klebsiella Pneumoniae Isolated From Farm Environments and Retail Products in Oklahoma." Journal of Food Protection, vol. 68, no. 10, 2005, pp. 2022-9.
Kim SH, Wei CI, Tzou YM, et al. Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. J Food Prot. 2005;68(10):2022-9.
Kim, S. H., Wei, C. I., Tzou, Y. M., & An, H. (2005). Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. Journal of Food Protection, 68(10), pp. 2022-9.
Kim SH, et al. Multidrug-resistant Klebsiella Pneumoniae Isolated From Farm Environments and Retail Products in Oklahoma. J Food Prot. 2005;68(10):2022-9. PubMed PMID: 16245702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. AU - Kim,Shin-Hee, AU - Wei,Cheng-I, AU - Tzou,Ywh-Min, AU - An,Haejung, PY - 2005/10/26/pubmed PY - 2005/11/11/medline PY - 2005/10/26/entrez SP - 2022 EP - 9 JF - Journal of food protection JO - J. Food Prot. VL - 68 IS - 10 N2 - Multidrug-resistant enteric bacteria were isolated from turkey, cattle, and chicken farms and retail meat products in Oklahoma. Among the isolated species, multidrug-resistant Klebsiella pneumoniae was prevalently isolated from most of the collected samples. Therefore, a total of 132 isolates of K. pneumoniae were characterized to understand their potential roles in the dissemination of antibiotic-resistance genes in the food chains. Multidrug-resistant K. pneumoniae was most frequently recovered from a turkey farm and ground turkey products among the tested samples. All isolates were resistant to ampicillin, tetracycline, streptomycin, gentamycin, and kanamycin. Class 1 integrons located in plasmids were identified as a common carrier of the aadA1 gene, encoding resistance to streptomycin and spectinomycin. Production of beta-lactamase in the K. pneumoniae isolates played a major role in the resistance to beta-lactam agents. Most isolates (96%) possessed bla(SHV1). Five strains were able to express both SHV-11 (pI 6.2) and TEM-1 (pI 5.2) beta-lactamase. Transfer of these antibiotic-resistance genes to Escherichia coli was demonstrated by transconjugation. The bacterial genomic DNA restriction patterns by pulsed-field gel electrophoresis showed that the same clones of multidrug-resistant K. pneumoniae remained in feathers, feed, feces, and drinking water in turkey environments, indicating the possible dissemination of antibiotic-resistance genes in the ecosystem and cross-contamination of antibiotic-resistant bacteria during processing and distribution of products. SN - 0362-028X UR - https://www.unboundmedicine.com/medline/citation/16245702/full_citation L2 - https://medlineplus.gov/foodborneillness.html DB - PRIME DP - Unbound Medicine ER -