Tags

Type your tag names separated by a space and hit enter

Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma.
J Food Prot 2005; 68(10):2022-9JF

Abstract

Multidrug-resistant enteric bacteria were isolated from turkey, cattle, and chicken farms and retail meat products in Oklahoma. Among the isolated species, multidrug-resistant Klebsiella pneumoniae was prevalently isolated from most of the collected samples. Therefore, a total of 132 isolates of K. pneumoniae were characterized to understand their potential roles in the dissemination of antibiotic-resistance genes in the food chains. Multidrug-resistant K. pneumoniae was most frequently recovered from a turkey farm and ground turkey products among the tested samples. All isolates were resistant to ampicillin, tetracycline, streptomycin, gentamycin, and kanamycin. Class 1 integrons located in plasmids were identified as a common carrier of the aadA1 gene, encoding resistance to streptomycin and spectinomycin. Production of beta-lactamase in the K. pneumoniae isolates played a major role in the resistance to beta-lactam agents. Most isolates (96%) possessed bla(SHV1). Five strains were able to express both SHV-11 (pI 6.2) and TEM-1 (pI 5.2) beta-lactamase. Transfer of these antibiotic-resistance genes to Escherichia coli was demonstrated by transconjugation. The bacterial genomic DNA restriction patterns by pulsed-field gel electrophoresis showed that the same clones of multidrug-resistant K. pneumoniae remained in feathers, feed, feces, and drinking water in turkey environments, indicating the possible dissemination of antibiotic-resistance genes in the ecosystem and cross-contamination of antibiotic-resistant bacteria during processing and distribution of products.

Authors+Show Affiliations

Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. kimshin@okstate.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16245702

Citation

Kim, Shin-Hee, et al. "Multidrug-resistant Klebsiella Pneumoniae Isolated From Farm Environments and Retail Products in Oklahoma." Journal of Food Protection, vol. 68, no. 10, 2005, pp. 2022-9.
Kim SH, Wei CI, Tzou YM, et al. Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. J Food Prot. 2005;68(10):2022-9.
Kim, S. H., Wei, C. I., Tzou, Y. M., & An, H. (2005). Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. Journal of Food Protection, 68(10), pp. 2022-9.
Kim SH, et al. Multidrug-resistant Klebsiella Pneumoniae Isolated From Farm Environments and Retail Products in Oklahoma. J Food Prot. 2005;68(10):2022-9. PubMed PMID: 16245702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multidrug-resistant Klebsiella pneumoniae isolated from farm environments and retail products in Oklahoma. AU - Kim,Shin-Hee, AU - Wei,Cheng-I, AU - Tzou,Ywh-Min, AU - An,Haejung, PY - 2005/10/26/pubmed PY - 2005/11/11/medline PY - 2005/10/26/entrez SP - 2022 EP - 9 JF - Journal of food protection JO - J. Food Prot. VL - 68 IS - 10 N2 - Multidrug-resistant enteric bacteria were isolated from turkey, cattle, and chicken farms and retail meat products in Oklahoma. Among the isolated species, multidrug-resistant Klebsiella pneumoniae was prevalently isolated from most of the collected samples. Therefore, a total of 132 isolates of K. pneumoniae were characterized to understand their potential roles in the dissemination of antibiotic-resistance genes in the food chains. Multidrug-resistant K. pneumoniae was most frequently recovered from a turkey farm and ground turkey products among the tested samples. All isolates were resistant to ampicillin, tetracycline, streptomycin, gentamycin, and kanamycin. Class 1 integrons located in plasmids were identified as a common carrier of the aadA1 gene, encoding resistance to streptomycin and spectinomycin. Production of beta-lactamase in the K. pneumoniae isolates played a major role in the resistance to beta-lactam agents. Most isolates (96%) possessed bla(SHV1). Five strains were able to express both SHV-11 (pI 6.2) and TEM-1 (pI 5.2) beta-lactamase. Transfer of these antibiotic-resistance genes to Escherichia coli was demonstrated by transconjugation. The bacterial genomic DNA restriction patterns by pulsed-field gel electrophoresis showed that the same clones of multidrug-resistant K. pneumoniae remained in feathers, feed, feces, and drinking water in turkey environments, indicating the possible dissemination of antibiotic-resistance genes in the ecosystem and cross-contamination of antibiotic-resistant bacteria during processing and distribution of products. SN - 0362-028X UR - https://www.unboundmedicine.com/medline/citation/16245702/full_citation L2 - http://jfoodprotection.org/doi/10.4315/0362-028x-68.10.2022?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -