DAPI as a useful stain for nuclear quantitation.Biotech Histochem. 1991; 66(6):297-302.BH
Abstract
A simple-to-use fluorescent stain, 4',6-diamidino-2-phenylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology. Following light microscopic analyses, the stained cells were processed for electron microscopy. Cells stained with DAPI showed no ultrastructural changes compared to the appearance of cells not stained with DAPI. DAPI staining allows multiple use of cells eliminating the need for duplicate samples.
MeSH
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Language
eng
PubMed ID
1725854
Citation
Tarnowski, B I., et al. "DAPI as a Useful Stain for Nuclear Quantitation." Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission, vol. 66, no. 6, 1991, pp. 297-302.
Tarnowski BI, Spinale FG, Nicholson JH. DAPI as a useful stain for nuclear quantitation. Biotech Histochem. 1991;66(6):297-302.
Tarnowski, B. I., Spinale, F. G., & Nicholson, J. H. (1991). DAPI as a useful stain for nuclear quantitation. Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission, 66(6), 297-302.
Tarnowski BI, Spinale FG, Nicholson JH. DAPI as a Useful Stain for Nuclear Quantitation. Biotech Histochem. 1991;66(6):297-302. PubMed PMID: 1725854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - DAPI as a useful stain for nuclear quantitation.
AU - Tarnowski,B I,
AU - Spinale,F G,
AU - Nicholson,J H,
PY - 1991/1/1/pubmed
PY - 1991/1/1/medline
PY - 1991/1/1/entrez
SP - 297
EP - 302
JF - Biotechnic & histochemistry : official publication of the Biological Stain Commission
JO - Biotech Histochem
VL - 66
IS - 6
N2 - A simple-to-use fluorescent stain, 4',6-diamidino-2-phenylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology. Following light microscopic analyses, the stained cells were processed for electron microscopy. Cells stained with DAPI showed no ultrastructural changes compared to the appearance of cells not stained with DAPI. DAPI staining allows multiple use of cells eliminating the need for duplicate samples.
SN - 1052-0295
UR - https://www.unboundmedicine.com/medline/citation/1725854/DAPI_as_a_useful_stain_for_nuclear_quantitation_
DB - PRIME
DP - Unbound Medicine
ER -
