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Testing for IgG class antibodies in celiac disease patients with selective IgA deficiency. A comparison of the diagnostic accuracy of 9 IgG anti-tissue transglutaminase, 1 IgG anti-gliadin and 1 IgG anti-deaminated gliadin peptide antibody assays.
Clin Chim Acta. 2007 Jul; 382(1-2):95-9.CC

Abstract

BACKGROUND

To evaluate the diagnostic characteristics of commercially available IgG anti-tTG assays in selective IgA deficiency (SIgAD), we tested different IgG anti-tTG methods and compared the results with those obtained from two other tests: one for IgG anti-gliadin (AGA) and one for IgG to deaminated gliadin peptides (DGP).

METHODS

20 CD patients with SIgAD and 113 controls (9 patients with SIgAD without CD; 54 patients with chronic liver disease; 50 healthy subjects) were tested with 9 IgG anti-tTG assays (2 of which are enriched with gliadin peptides), one IgG AGA assay and one IgG anti-DGP assay.

RESULTS

Using optimal cutoffs as determined by ROC curves, the sensitivity of IgG anti-tTG methods ranged from 75% (1 kit) to 95% (7 kits) and the specificity from 94% (1 kit) to 100% (5 kits). Sensitivity and specificity were 40% and 87% for IgG AGA, and 80% and 98% for IgG anti-DGP, respectively.

CONCLUSIONS

All IgG anti-tTG methods evaluated are reliable serologic assays for the diagnosis of CD in patients with SIgAD and perform better than the gliadin-based assays used in this study. The tests containing both tTG and gliadinic peptides are burdened by a lower specificity than the anti-tTG assays.

Authors+Show Affiliations

Villalta D
Immunologia Clinica e Virologia, AO S Maria degli Angeli, Via Montereale 24, Pordenone, Italy. danilo.villalta@aopn.fvg.it
Alessio MG
No affiliation info available
Tampoia M
No affiliation info available
Tonutti E
No affiliation info available
Brusca I
No affiliation info available
Bagnasco M
No affiliation info available
Pesce G
No affiliation info available
Stella S
No affiliation info available
Bizzaro N
No affiliation info available

MeSH

AdolescentAdultAutoantibodiesCeliac DiseaseChildChild, PreschoolEnzyme-Linked Immunosorbent AssayFemaleGliadinHumansIgA DeficiencyImmunoglobulin GMaleMiddle AgedReagent Kits, DiagnosticSensitivity and SpecificityTransglutaminases

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

17490629

Citation

Villalta, Danilo, et al. "Testing for IgG Class Antibodies in Celiac Disease Patients With Selective IgA Deficiency. a Comparison of the Diagnostic Accuracy of 9 IgG Anti-tissue Transglutaminase, 1 IgG Anti-gliadin and 1 IgG Anti-deaminated Gliadin Peptide Antibody Assays." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 382, no. 1-2, 2007, pp. 95-9.
Villalta D, Alessio MG, Tampoia M, et al. Testing for IgG class antibodies in celiac disease patients with selective IgA deficiency. A comparison of the diagnostic accuracy of 9 IgG anti-tissue transglutaminase, 1 IgG anti-gliadin and 1 IgG anti-deaminated gliadin peptide antibody assays. Clin Chim Acta. 2007;382(1-2):95-9.
Villalta, D., Alessio, M. G., Tampoia, M., Tonutti, E., Brusca, I., Bagnasco, M., Pesce, G., Stella, S., & Bizzaro, N. (2007). Testing for IgG class antibodies in celiac disease patients with selective IgA deficiency. A comparison of the diagnostic accuracy of 9 IgG anti-tissue transglutaminase, 1 IgG anti-gliadin and 1 IgG anti-deaminated gliadin peptide antibody assays. Clinica Chimica Acta; International Journal of Clinical Chemistry, 382(1-2), 95-9.
Villalta D, et al. Testing for IgG Class Antibodies in Celiac Disease Patients With Selective IgA Deficiency. a Comparison of the Diagnostic Accuracy of 9 IgG Anti-tissue Transglutaminase, 1 IgG Anti-gliadin and 1 IgG Anti-deaminated Gliadin Peptide Antibody Assays. Clin Chim Acta. 2007;382(1-2):95-9. PubMed PMID: 17490629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Testing for IgG class antibodies in celiac disease patients with selective IgA deficiency. A comparison of the diagnostic accuracy of 9 IgG anti-tissue transglutaminase, 1 IgG anti-gliadin and 1 IgG anti-deaminated gliadin peptide antibody assays. AU - Villalta,Danilo, AU - Alessio,Maria Grazia, AU - Tampoia,Marilina, AU - Tonutti,Elio, AU - Brusca,Ignazio, AU - Bagnasco,Marcello, AU - Pesce,Giampaola, AU - Stella,Sergio, AU - Bizzaro,Nicola, Y1 - 2007/04/13/ PY - 2007/02/20/received PY - 2007/03/30/revised PY - 2007/03/30/accepted PY - 2007/5/11/pubmed PY - 2007/8/19/medline PY - 2007/5/11/entrez SP - 95 EP - 9 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 382 IS - 1-2 N2 - BACKGROUND: To evaluate the diagnostic characteristics of commercially available IgG anti-tTG assays in selective IgA deficiency (SIgAD), we tested different IgG anti-tTG methods and compared the results with those obtained from two other tests: one for IgG anti-gliadin (AGA) and one for IgG to deaminated gliadin peptides (DGP). METHODS: 20 CD patients with SIgAD and 113 controls (9 patients with SIgAD without CD; 54 patients with chronic liver disease; 50 healthy subjects) were tested with 9 IgG anti-tTG assays (2 of which are enriched with gliadin peptides), one IgG AGA assay and one IgG anti-DGP assay. RESULTS: Using optimal cutoffs as determined by ROC curves, the sensitivity of IgG anti-tTG methods ranged from 75% (1 kit) to 95% (7 kits) and the specificity from 94% (1 kit) to 100% (5 kits). Sensitivity and specificity were 40% and 87% for IgG AGA, and 80% and 98% for IgG anti-DGP, respectively. CONCLUSIONS: All IgG anti-tTG methods evaluated are reliable serologic assays for the diagnosis of CD in patients with SIgAD and perform better than the gliadin-based assays used in this study. The tests containing both tTG and gliadinic peptides are burdened by a lower specificity than the anti-tTG assays. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/17490629/full_citation DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓16]

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