TY - JOUR T1 - DNA packaging-associated hyper-capsid expansion of bacteriophage t3. AU - Serwer,Philip, AU - Wright,Elena T, AU - Hakala,Kevin, AU - Weintraub,Susan T, AU - Su,Min, AU - Jiang,Wen, Y1 - 2010/02/01/ PY - 2009/10/21/received PY - 2010/01/20/revised PY - 2010/01/26/accepted PY - 2010/2/4/entrez PY - 2010/2/4/pubmed PY - 2010/3/26/medline SP - 361 EP - 74 JF - Journal of molecular biology JO - J Mol Biol VL - 397 IS - 2 N2 - Evidence that in vivo bacteriophage T3 DNA packaging includes capsid hyper-expansion that is triggered by lengthening of incompletely packaged DNA (ipDNA) is presented here. This evidence includes observation that some of the longer ipDNAs in T3-infected cells are packaged in ipDNA-containing capsids with hyper-expanded outer shells (HE ipDNA-capsids). In addition, artificially induced hyper-expansion is observed for the outer shell of a DNA-free capsid. Detection and characterization of HE ipDNA-capsids are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by structure determination with electron microscopy and protein identification with SDS-PAGE/mass spectrometry. After expulsion from HE ipDNA-capsids, ipDNA forms sharp bands during gel electrophoresis. The following hypotheses are presented: (1) T3 has evolved feedback-initiated, ATP-driven capsid contraction/hyper-expansion cycles that accelerate DNA packaging when packaging is slowed by increase in the packaging-resisting force of the ipDNA and (2) each gel electrophoretic ipDNA band reflects a contraction/hyper-expansion cycle. SN - 1089-8638 UR - https://www.unboundmedicine.com/medline/citation/20122936/DNA_packaging_associated_hyper_capsid_expansion_of_bacteriophage_t3_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(10)00113-0 DB - PRIME DP - Unbound Medicine ER -