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Flavonoid glycosides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase.
PLoS One 2012; 7(3):e32214Plos

Abstract

Legumes and the polyphenolic compounds present in them have gained a lot of interest due to their beneficial health implications. Dietary polyphenolic compounds, especially flavonoids, exert antioxidant properties and are potent inhibitors of xanthine oxidase (XO) activity. XO is the main contributor of free radicals during exercise but it is also involved in pathogenesis of several diseases such as vascular disorders, cancer and gout. In order to discover new natural, dietary XO inhibitors, some polyphenolic fractions and pure compounds isolated from two legume plant extracts were tested for their effects on XO activity. The fractions isolated from both Vicia faba and Lotus edulis plant extracts were potent inhibitors of XO with IC(50) values range from 40-135 µg/mL and 55-260 µg/mL, respectively. All the pure polyphenolic compounds inhibited XO and their K(i) values ranged from 13-767 µM. Ten of the compounds followed the non competitive inhibitory model whereas one of them was a competitive inhibitor. These findings indicate that flavonoid isolates from legume plant extracts are novel, natural XO inhibitors. Their mode of action is under investigation in order to examine their potential in drug design for diseases related to overwhelming XO action.

Authors+Show Affiliations

Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22396752

Citation

Spanou, Chrysoula, et al. "Flavonoid Glycosides Isolated From Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase." PloS One, vol. 7, no. 3, 2012, pp. e32214.
Spanou C, Veskoukis AS, Kerasioti T, et al. Flavonoid glycosides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase. PLoS ONE. 2012;7(3):e32214.
Spanou, C., Veskoukis, A. S., Kerasioti, T., Kontou, M., Angelis, A., Aligiannis, N., ... Kouretas, D. (2012). Flavonoid glycosides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase. PloS One, 7(3), pp. e32214. doi:10.1371/journal.pone.0032214.
Spanou C, et al. Flavonoid Glycosides Isolated From Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase. PLoS ONE. 2012;7(3):e32214. PubMed PMID: 22396752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavonoid glycosides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase. AU - Spanou,Chrysoula, AU - Veskoukis,Aristidis S, AU - Kerasioti,Thalia, AU - Kontou,Maria, AU - Angelis,Apostolos, AU - Aligiannis,Nektarios, AU - Skaltsounis,Alexios-Leandros, AU - Kouretas,Dimitrios, Y1 - 2012/03/02/ PY - 2011/11/16/received PY - 2012/01/25/accepted PY - 2012/3/8/entrez PY - 2012/3/8/pubmed PY - 2012/7/24/medline SP - e32214 EP - e32214 JF - PloS one JO - PLoS ONE VL - 7 IS - 3 N2 - Legumes and the polyphenolic compounds present in them have gained a lot of interest due to their beneficial health implications. Dietary polyphenolic compounds, especially flavonoids, exert antioxidant properties and are potent inhibitors of xanthine oxidase (XO) activity. XO is the main contributor of free radicals during exercise but it is also involved in pathogenesis of several diseases such as vascular disorders, cancer and gout. In order to discover new natural, dietary XO inhibitors, some polyphenolic fractions and pure compounds isolated from two legume plant extracts were tested for their effects on XO activity. The fractions isolated from both Vicia faba and Lotus edulis plant extracts were potent inhibitors of XO with IC(50) values range from 40-135 µg/mL and 55-260 µg/mL, respectively. All the pure polyphenolic compounds inhibited XO and their K(i) values ranged from 13-767 µM. Ten of the compounds followed the non competitive inhibitory model whereas one of them was a competitive inhibitor. These findings indicate that flavonoid isolates from legume plant extracts are novel, natural XO inhibitors. Their mode of action is under investigation in order to examine their potential in drug design for diseases related to overwhelming XO action. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22396752/full_citation L2 - http://dx.plos.org/10.1371/journal.pone.0032214 DB - PRIME DP - Unbound Medicine ER -