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Early detection of high oxidative activity in patients with adenomatous intestinal polyps and colorectal adenocarcinoma: myeloperoxidase and oxidized low-density lipoprotein in serum as new markers of oxidative stress in colorectal cancer.

Abstract

OBJECTIVES

To detect whether signs of oxidative stress appear at early stages of colorectal adenocarcinoma (CRC), particularly in the polyp stage. We also aimed to evaluate the specific entities myeloperoxidase (MPO) and oxidized low-density lipoprotein (oxLDL) as novel markers of oxidation in the plasma of patients with CRC and to study the relationship between oxidative status in plasma and patient survival.

METHODS

We assayed serum or plasma specimens from healthy control subjects (n = 14), from patients with intestinal polyps (n = 39), and from patients with CRC (n = 128) to calculate the modified oxidative balance score (MOBS) using several serum markers (β-carotene, lycopene, vitamin A, vitamin E, MPO, and oxLDL). We also assayed the levels of C-reactive protein (CRP) and obtained lipid profiles. Finally, we studied the survival of patients in relationship to oxidative status (antioxidants and pro-oxidants) and inflammation markers, and added theses data to the lipid profile for each patient.

RESULTS

Oxidative stress levels increased as disease stage advanced. This increase was detected early in the polyp stage, before polyps progressed to cancer, and could be measured by the increase of such new markers as MPO and oxLDL, the decrease in antioxidants, and the MOBS value. Higher levels of oxidation correlated with lower survival.

CONCLUSION

The oxidation process, which can cause mutations leading to CRC, begins development in the polyp stage. This process may be detected early by monitoring serum markers such as MPO and oxLDL.

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  • Authors+Show Affiliations

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain pmpeius@hotmail.com.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Nutrition, School of Medicine, University of Valladolid, Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Biochemistry, Molecular Biology, and Physiology, Biology and Genetics Institute, University of Valladolid-Superior Council of Scientific Investigations (CSIC), Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Biochemistry, Molecular Biology, and Physiology, Biology and Genetics Institute, University of Valladolid-Superior Council of Scientific Investigations (CSIC), Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

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    Department of Clinical Laboratory, Clinical Hospital, University of Valladolid, Valladolid, Spain.

    Río Hortega University Hospital, Valladolid, Spain.

    Source

    Laboratory medicine 46:2 2015 pg 123-35

    MeSH

    Adenomatous Polyposis Coli
    Aged
    Aged, 80 and over
    Analysis of Variance
    C-Reactive Protein
    Cohort Studies
    Colorectal Neoplasms
    Early Detection of Cancer
    Female
    Humans
    Lipoproteins, LDL
    Male
    Middle Aged
    Peroxidase

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    25918191

    Citation

    Crespo-Sanjuán, Jesús, et al. "Early Detection of High Oxidative Activity in Patients With Adenomatous Intestinal Polyps and Colorectal Adenocarcinoma: Myeloperoxidase and Oxidized Low-density Lipoprotein in Serum as New Markers of Oxidative Stress in Colorectal Cancer." Laboratory Medicine, vol. 46, no. 2, 2015, pp. 123-35.
    Crespo-Sanjuán J, Calvo-Nieves MD, Aguirre-Gervás B, et al. Early detection of high oxidative activity in patients with adenomatous intestinal polyps and colorectal adenocarcinoma: myeloperoxidase and oxidized low-density lipoprotein in serum as new markers of oxidative stress in colorectal cancer. Lab Med. 2015;46(2):123-35.
    Crespo-Sanjuán, J., Calvo-Nieves, M. D., Aguirre-Gervás, B., Herreros-Rodríguez, J., Velayos-Jiménez, B., Castro-Alija, M. J., ... Garrote-Adrados, J. A. (2015). Early detection of high oxidative activity in patients with adenomatous intestinal polyps and colorectal adenocarcinoma: myeloperoxidase and oxidized low-density lipoprotein in serum as new markers of oxidative stress in colorectal cancer. Laboratory Medicine, 46(2), pp. 123-35. doi:10.1309/LMZJJU6BC86WUDHW.
    Crespo-Sanjuán J, et al. Early Detection of High Oxidative Activity in Patients With Adenomatous Intestinal Polyps and Colorectal Adenocarcinoma: Myeloperoxidase and Oxidized Low-density Lipoprotein in Serum as New Markers of Oxidative Stress in Colorectal Cancer. Lab Med. 2015;46(2):123-35. PubMed PMID: 25918191.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Early detection of high oxidative activity in patients with adenomatous intestinal polyps and colorectal adenocarcinoma: myeloperoxidase and oxidized low-density lipoprotein in serum as new markers of oxidative stress in colorectal cancer. AU - Crespo-Sanjuán,Jesús, AU - Calvo-Nieves,María D, AU - Aguirre-Gervás,Beatriz, AU - Herreros-Rodríguez,José, AU - Velayos-Jiménez,Benito, AU - Castro-Alija,María J, AU - Muñoz-Moreno,María F, AU - Sánchez,Diego, AU - Zamora-González,Nuria, AU - Bajo-Grañeras,Raquel, AU - García-Centeno,Rosa M, AU - Largo Cabrerizo,María E, AU - Bustamante,María R, AU - Garrote-Adrados,José A, PY - 2015/4/29/entrez PY - 2015/4/29/pubmed PY - 2016/7/12/medline KW - LDL oxidized KW - antioxidants KW - cancer biomarkers KW - colorectal cancer KW - myeloperoxidase KW - oxidative stress SP - 123 EP - 35 JF - Laboratory medicine JO - Lab Med VL - 46 IS - 2 N2 - OBJECTIVES: To detect whether signs of oxidative stress appear at early stages of colorectal adenocarcinoma (CRC), particularly in the polyp stage. We also aimed to evaluate the specific entities myeloperoxidase (MPO) and oxidized low-density lipoprotein (oxLDL) as novel markers of oxidation in the plasma of patients with CRC and to study the relationship between oxidative status in plasma and patient survival. METHODS: We assayed serum or plasma specimens from healthy control subjects (n = 14), from patients with intestinal polyps (n = 39), and from patients with CRC (n = 128) to calculate the modified oxidative balance score (MOBS) using several serum markers (β-carotene, lycopene, vitamin A, vitamin E, MPO, and oxLDL). We also assayed the levels of C-reactive protein (CRP) and obtained lipid profiles. Finally, we studied the survival of patients in relationship to oxidative status (antioxidants and pro-oxidants) and inflammation markers, and added theses data to the lipid profile for each patient. RESULTS: Oxidative stress levels increased as disease stage advanced. This increase was detected early in the polyp stage, before polyps progressed to cancer, and could be measured by the increase of such new markers as MPO and oxLDL, the decrease in antioxidants, and the MOBS value. Higher levels of oxidation correlated with lower survival. CONCLUSION: The oxidation process, which can cause mutations leading to CRC, begins development in the polyp stage. This process may be detected early by monitoring serum markers such as MPO and oxLDL. SN - 1943-7730 UR - https://www.unboundmedicine.com/medline/citation/25918191/full_citation L2 - https://academic.oup.com/labmed/article-lookup/doi/10.1309/LMZJJU6BC86WUDHW DB - PRIME DP - Unbound Medicine ER -