Tags

Type your tag names separated by a space and hit enter

Anti-Aging Potentials of Methylene Blue for Human Skin Longevity.
Sci Rep. 2017 May 30; 7(1):2475.SR

Abstract

Oxidative stress is the major cause of skin aging that includes wrinkles, pigmentation, and weakened wound healing ability. Application of antioxidants in skin care is well accepted as an effective approach to delay the skin aging process. Methylene blue (MB), a traditional mitochondrial-targeting antioxidant, showed a potent ROS scavenging efficacy in cultured human skin fibroblasts derived from healthy donors and from patients with progeria, a genetic premature aging disease. In comparison with other widely used general and mitochondrial-targeting antioxidants, we found that MB was more effective in stimulating skin fibroblast proliferation and delaying cellular senescence. The skin irritation test, performed on an in vitro reconstructed 3D human skin model, indicated that MB was safe for long-term use, and did not cause irritation even at high concentrations. Application of MB to this 3D skin model further demonstrated that MB improved skin viability, promoted wound healing and increased skin hydration and dermis thickness. Gene expression analysis showed that MB treatment altered the expression of a subset of extracellular matrix proteins in the skin, including upregulation of elastin and collagen 2A1, two essential components for healthy skin. Altogether, our study suggests that MB has a great potential for skin care.

Links

PMC Free Full Text

Authors+Show Affiliations

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.

Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA. kcao@umd.edu.

MeSH

AntioxidantsCell ProliferationCellular SenescenceCollagenElastinFibroblastsHumansLongevityMethylene BlueMitochondriaOxidative StressSkinSkin AgingWound Healing

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28559565

Citation

Xiong, Zheng-Mei, et al. "Anti-Aging Potentials of Methylene Blue for Human Skin Longevity." Scientific Reports, vol. 7, no. 1, 2017, p. 2475.

Xiong ZM, O'Donovan M, Sun L, et al. Anti-Aging Potentials of Methylene Blue for Human Skin Longevity. Sci Rep. 2017;7(1):2475.

Xiong, Z. M., O'Donovan, M., Sun, L., Choi, J. Y., Ren, M., & Cao, K. (2017). Anti-Aging Potentials of Methylene Blue for Human Skin Longevity. Scientific Reports, 7(1), 2475. https://doi.org/10.1038/s41598-017-02419-3

Xiong ZM, et al. Anti-Aging Potentials of Methylene Blue for Human Skin Longevity. Sci Rep. 2017 05 30;7(1):2475. PubMed PMID: 28559565.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Anti-Aging Potentials of Methylene Blue for Human Skin Longevity. AU - Xiong,Zheng-Mei, AU - O'Donovan,Mike, AU - Sun,Linlin, AU - Choi,Ji Young, AU - Ren,Margaret, AU - Cao,Kan, Y1 - 2017/05/30/ PY - 2017/01/16/received PY - 2017/04/11/accepted PY - 2017/6/1/entrez PY - 2017/6/1/pubmed PY - 2018/12/14/medline SP - 2475 EP - 2475 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - Oxidative stress is the major cause of skin aging that includes wrinkles, pigmentation, and weakened wound healing ability. Application of antioxidants in skin care is well accepted as an effective approach to delay the skin aging process. Methylene blue (MB), a traditional mitochondrial-targeting antioxidant, showed a potent ROS scavenging efficacy in cultured human skin fibroblasts derived from healthy donors and from patients with progeria, a genetic premature aging disease. In comparison with other widely used general and mitochondrial-targeting antioxidants, we found that MB was more effective in stimulating skin fibroblast proliferation and delaying cellular senescence. The skin irritation test, performed on an in vitro reconstructed 3D human skin model, indicated that MB was safe for long-term use, and did not cause irritation even at high concentrations. Application of MB to this 3D skin model further demonstrated that MB improved skin viability, promoted wound healing and increased skin hydration and dermis thickness. Gene expression analysis showed that MB treatment altered the expression of a subset of extracellular matrix proteins in the skin, including upregulation of elastin and collagen 2A1, two essential components for healthy skin. Altogether, our study suggests that MB has a great potential for skin care. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28559565/Anti-Aging_Potentials_of_Methylene_Blue_for_Human_Skin_Longevity. L2 - https://doi.org/10.1038/s41598-017-02419-3 DB - PRIME DP - Unbound Medicine ER -