TY - JOUR T1 - Physiological, biochemical and molecular responses of Mentha aquatica L. to manganese. AU - Nazari,Mehrdad, AU - Zarinkamar,Fatemeh, AU - Soltani,Bahram Mohammad, Y1 - 2017/08/04/ PY - 2017/06/09/received PY - 2017/08/01/revised PY - 2017/08/03/accepted PY - 2017/10/23/pubmed PY - 2018/5/31/medline PY - 2017/10/23/entrez KW - Antioxidative responses KW - Essential oils KW - Gene expression KW - Manganese KW - Terpenoid biosynthetic genes KW - Water mint SP - 202 EP - 212 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 120 N2 - Mentha aquatica is an aromatic herb which possesses valuable terpenoids constituents. Here, we intended to evaluate the effects of the different manganese (Mn) concentrations on the physiological, biochemical and molecular responses in M. aquatica. Basic Hoagland's solution (control), 40, 80, and 160 μM of Mn supplied as MnSO4·H2O were applied to the nutrient solution. The results indicated that the different concentrations of Mn differently affected the physiological, biochemical and molecular responses in M. aquatica. The growth parameters (biomass and photosynthetic pigments) and expression levels of β-caryophyllene synthase (CPS), limonene synthase (Ls), geranyl diphosphate synthase (Gpps), and menthofuran synthase (Mfs) genes were increased at the moderate Mn concentrations (40 and 80 μM) and began to decrease at the higher levels. However, the contents of anthocyanins, flavonoids, malonaldehyde (MDA) and hydrogen peroxide (H2O2), Mn accumulation, activities of antioxidant enzymes, yield of essential oils and the expression levels of 1-Deoxy d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Ippi) genes were gradually increased with increasing concentration of Mn in the nutrient solution. Also, the content and chemical composition of terpenoid constituents were altered in the Mn-treated plants. Here, we suggest that the application of external Mn in nutrient solution elevates the growth and expression levels of the genes that are involved in the terpenoid biosynthesis pathway in M. aquatica. Nevertheless, the extent and stability of these growth and gene expression elevation are varied among the different Mn treatments. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/29055856 DB - PRIME DP - Unbound Medicine ER -