TY - JOUR T1 - The Expanding Toolkit of Translating Ribosome Affinity Purification. A1 - Dougherty,Joseph D, PY - 2017/08/07/received PY - 2017/10/16/revised PY - 2017/11/03/accepted PY - 2017/12/15/entrez PY - 2017/12/15/pubmed PY - 2018/1/20/medline KW - RNASeq KW - local translation KW - ribosome profiling KW - translating ribosome affinity purification KW - translation regulation SP - 12079 EP - 12087 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 37 IS - 50 N2 - Translating ribosome affinity purification is a method initially developed for profiling mRNA from genetically defined cell types in complex tissues. It has been applied both to identify target molecules in cell types that are important for controlling a variety of behaviors in the brain, and to understand the molecular consequences on those cells due to experimental manipulations, ranging from drugs of abuse to disease-causing mutations. Since its inception, a variety of methodological advances are opening new avenues of investigation. These advances include a variety of new methods for targeting cells for translating ribosome affinity purification by features such as their projections or activity, additional tags and mouse reagents increasing the flexibility of the system, and new modifications of the method specifically focused on studying the regulation of translation. The latter includes methods to assess cell type-specific regulation of translation in specific subcellular compartments. Here, I provide a summary of these recent advances and resources, highlighting both new experimental opportunities and areas for future technical development. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/29237735/The_Expanding_Toolkit_of_Translating_Ribosome_Affinity_Purification_ DB - PRIME DP - Unbound Medicine ER -