Transcriptome Sequencing: RNA-Seq.
Methods Mol Biol. 2018; 1754:15-27.MM

Abstract

RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of different platforms are not exactly the same; each platform has its own kit to meet the special requirements of the instrument design.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang H

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center for Genetics and Development, Shanghai Jiaotong University, Shanghai, 200240, China.

He L

Cai L

MeSH

Alternative SplicingBase SequenceData AnalysisGene Expression ProfilingGene FusionHigh-Throughput Nucleotide SequencingRNASequence Analysis, RNATranscriptome

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29536435

Citation

Zhang, Hong, et al. "Transcriptome Sequencing: RNA-Seq." Methods in Molecular Biology (Clifton, N.J.), vol. 1754, 2018, pp. 15-27.

Zhang H, He L, Cai L. Transcriptome Sequencing: RNA-Seq. Methods Mol Biol. 2018;1754:15-27.

Zhang, H., He, L., & Cai, L. (2018). Transcriptome Sequencing: RNA-Seq. Methods in Molecular Biology (Clifton, N.J.), 1754, 15-27. https://doi.org/10.1007/978-1-4939-7717-8_2

Zhang H, He L, Cai L. Transcriptome Sequencing: RNA-Seq. Methods Mol Biol. 2018;1754:15-27. PubMed PMID: 29536435.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Transcriptome Sequencing: RNA-Seq. AU - Zhang,Hong, AU - He,Lin, AU - Cai,Lei, PY - 2018/3/15/entrez PY - 2018/3/15/pubmed PY - 2019/1/29/medline KW - Data analysis KW - Library construction KW - Messenger RNA KW - Next-generation sequencing KW - RNA sequencing SP - 15 EP - 27 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1754 N2 - RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of different platforms are not exactly the same; each platform has its own kit to meet the special requirements of the instrument design. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/29536435/Transcriptome_Sequencing:_RNA_Seq_ DB - PRIME DP - Unbound Medicine ER -

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Transcriptome Sequencing: RNA-Seq.Methods Mol Biol. 2018; 1754:15-27.MM