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An "On-Off" Electrochemiluminescence Aptasensor for Ochratoxin A Detection Based on CdS-Apt and Ru(bpy)32.
Langmuir. 2025 Jul 08; 41(26):16857-16866.L

Abstract

An "on-off" electrochemiluminescence (ECL) aptasensor was proposed for the highly sensitive and selective detection of ochratoxin A (OTA) in cereals. The substrate nanomaterial employed in the ECL experiments was titanium carbide (Ti3C2Tx) MXene, while cadmium sulfide quantum dots (CdS QDs) were used as coreactants of the ECL reagent tris (2,2'-bipyridyl) ruthenium(II) (Ru(bpy)32+). Compared to tripropylamine, a more toxic conventional coreactant, CdS QDs can react efficiently with Ru(bpy)32+ in the ECL reaction, and the reaction principles of both were also investigated. The effective connection between CdS QDs and Ru(bpy)32+ via the aptamer resulted in a shortened electron transfer distance, which in turn led to an improvement in ECL intensity and the achievement of the first "on" state. The introduction of OTA resulted in the loss of the effective connection between the CdS QDs and Ru(bpy)32+, which caused the sensor to enter an "off" state. The high ECL intensity of the sensor in the "on" state, in contrast with the low ECL intensity of the "off" state, resulted in an increase in the sensitivity and stability of the sensor. The established aptasensor exhibited a low limit of detection (LOD) of 0.03 ng/mL within a linear range of 0.1 ∼ 100 ng/mL, with spiked recoveries ranging from 93.7% to 105.1%, showing good selectivity, stability, and reproducibility for the detection of OTA in cereals.

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Authors+Show Affiliations

Sang M
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Han Y
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Zhang H
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Li Z
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Zhou Q
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Meng X
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Zhang Y
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Guo Y
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Sun X
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Usmanova SR
Chemistry of Biopolymers Lab, V.I. Nikitin Institute of Chemistry National Academy of Sciences of Tajikistan, Aini 299/2, Dushanbe 734063, Republic of Tajikistan.
Bobokalonov J
Chemistry of Biopolymers Lab, V.I. Nikitin Institute of Chemistry National Academy of Sciences of Tajikistan, Aini 299/2, Dushanbe 734063, Republic of Tajikistan.
Zhao W
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.

MeSH

OchratoxinsCadmium CompoundsSulfidesQuantum DotsAptamers, NucleotideLuminescent MeasurementsElectrochemical TechniquesOrganometallic CompoundsLimit of DetectionBiosensing TechniquesEdible Grain

Pub Type(s)

Journal Article

Language

eng

PubMed ID

40561070

Citation

Sang, Maosheng, et al. "An "On-Off" Electrochemiluminescence Aptasensor for Ochratoxin a Detection Based On CdS-Apt and Ru(bpy)32." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 41, no. 26, 2025, pp. 16857-16866.
Sang M, Han Y, Zhang H, et al. An "On-Off" Electrochemiluminescence Aptasensor for Ochratoxin A Detection Based on CdS-Apt and Ru(bpy)32. Langmuir. 2025;41(26):16857-16866.
Sang, M., Han, Y., Zhang, H., Li, Z., Zhou, Q., Meng, X., Zhang, Y., Guo, Y., Sun, X., Usmanova, S. R., Bobokalonov, J., & Zhao, W. (2025). An "On-Off" Electrochemiluminescence Aptasensor for Ochratoxin A Detection Based on CdS-Apt and Ru(bpy)32. Langmuir : the ACS Journal of Surfaces and Colloids, 41(26), 16857-16866. https://doi.org/10.1021/acs.langmuir.5c01006
Sang M, et al. An "On-Off" Electrochemiluminescence Aptasensor for Ochratoxin a Detection Based On CdS-Apt and Ru(bpy)32. Langmuir. 2025 Jul 8;41(26):16857-16866. PubMed PMID: 40561070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An "On-Off" Electrochemiluminescence Aptasensor for Ochratoxin A Detection Based on CdS-Apt and Ru(bpy)32. AU - Sang,Maosheng, AU - Han,Yang, AU - Zhang,Heng, AU - Li,Zhongyu, AU - Zhou,Quanlong, AU - Meng,Xiaoya, AU - Zhang,Yuhao, AU - Guo,Yemin, AU - Sun,Xia, AU - Usmanova,Surayo Rakhmatzhanovna, AU - Bobokalonov,Jamshed, AU - Zhao,Wenping, Y1 - 2025/06/25/ PY - 2025/7/8/medline PY - 2025/6/25/pubmed PY - 2025/6/25/entrez SP - 16857 EP - 16866 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 41 IS - 26 N2 - An "on-off" electrochemiluminescence (ECL) aptasensor was proposed for the highly sensitive and selective detection of ochratoxin A (OTA) in cereals. The substrate nanomaterial employed in the ECL experiments was titanium carbide (Ti3C2Tx) MXene, while cadmium sulfide quantum dots (CdS QDs) were used as coreactants of the ECL reagent tris (2,2'-bipyridyl) ruthenium(II) (Ru(bpy)32+). Compared to tripropylamine, a more toxic conventional coreactant, CdS QDs can react efficiently with Ru(bpy)32+ in the ECL reaction, and the reaction principles of both were also investigated. The effective connection between CdS QDs and Ru(bpy)32+ via the aptamer resulted in a shortened electron transfer distance, which in turn led to an improvement in ECL intensity and the achievement of the first "on" state. The introduction of OTA resulted in the loss of the effective connection between the CdS QDs and Ru(bpy)32+, which caused the sensor to enter an "off" state. The high ECL intensity of the sensor in the "on" state, in contrast with the low ECL intensity of the "off" state, resulted in an increase in the sensitivity and stability of the sensor. The established aptasensor exhibited a low limit of detection (LOD) of 0.03 ng/mL within a linear range of 0.1 ∼ 100 ng/mL, with spiked recoveries ranging from 93.7% to 105.1%, showing good selectivity, stability, and reproducibility for the detection of OTA in cereals. SN - 1520-5827 UR - https://www.unboundmedicine.com/medline/citation/40561070/An_ DB - PRIME DP - Unbound Medicine ER -

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