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Estrogen receptor beta is involved in skeletal muscle hypertrophy induced by the phytoecdysteroid ecdysterone.
Mol Nutr Food Res. 2014 Sep; 58(9):1861-72.MN

Abstract

SCOPE

The phytoectysteroid ecdysterone (Ecdy) was reported to stimulate protein synthesis and enhance physical performance. The aim of this study was to investigate underlying molecular mechanisms particularly the role of ER beta (ERβ).

RESULTS

In male rats, Ecdy treatment increased muscle fiber size, serum IGF-1 increased, and corticosteron and 17β-estradiol (E2) decreased. In differentiated C2C12 myoblastoma cells, treatment with Ecdy, dihydrotestosterone, IGF-1 but also E2 results in hypertrophy. Hypertrophy induced by E2 and Ecdy could be antagonized with an antiestrogen but not by an antiandrogen. In HEK293 cells transfected with ER alpha (ERα) or ERβ, Ecdy treatment transactivated a reporter gene. To elucidate the role of ERβ in Ecdy-mediated muscle hypertrophy, C2C12 myotubes were treated with ERα (ALPHA) and ERβ (BETA) selective ligands. Ecdy and BETA treatment but not ALPHA induced hypertrophy. The effect of Ecdy, E2, and BETA could be antagonized by an ERβ-selective antagonist (ANTIBETA). In summary, our results indicate that ERβ is involved in the mediation of the anabolic activity of the Ecdy.

CONCLUSION

These findings provide new therapeutic perspectives for the treatment of muscle injuries, sarcopenia, and cachectic disease, but also imply that such a substance could be abused for doping purposes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Parr MK
Center for Preventive Doping Research, German Sport University, Cologne, Germany; Institute of Pharmacy, Freie Universität Berlin, Germany.
Zhao P
No affiliation info available
Haupt O
No affiliation info available
Ngueu ST
No affiliation info available
Hengevoss J
No affiliation info available
Fritzemeier KH
No affiliation info available
Piechotta M
No affiliation info available
Schlörer N
No affiliation info available
Muhn P
No affiliation info available
Zheng WY
No affiliation info available
Xie MY
No affiliation info available
Diel P
No affiliation info available

MeSH

Anabolic AgentsAnimalsCell LineDexamethasoneEcdysteroneEstradiolEstrogen Receptor betaHEK293 CellsHumansHypertrophyMaleMuscle Fibers, SkeletalMuscle, SkeletalRats, Wistar

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24974955

Citation

Parr, Maria Kristina, et al. "Estrogen Receptor Beta Is Involved in Skeletal Muscle Hypertrophy Induced By the Phytoecdysteroid Ecdysterone." Molecular Nutrition & Food Research, vol. 58, no. 9, 2014, pp. 1861-72.
Parr MK, Zhao P, Haupt O, et al. Estrogen receptor beta is involved in skeletal muscle hypertrophy induced by the phytoecdysteroid ecdysterone. Mol Nutr Food Res. 2014;58(9):1861-72.
Parr, M. K., Zhao, P., Haupt, O., Ngueu, S. T., Hengevoss, J., Fritzemeier, K. H., Piechotta, M., Schlörer, N., Muhn, P., Zheng, W. Y., Xie, M. Y., & Diel, P. (2014). Estrogen receptor beta is involved in skeletal muscle hypertrophy induced by the phytoecdysteroid ecdysterone. Molecular Nutrition & Food Research, 58(9), 1861-72. https://doi.org/10.1002/mnfr.201300806
Parr MK, et al. Estrogen Receptor Beta Is Involved in Skeletal Muscle Hypertrophy Induced By the Phytoecdysteroid Ecdysterone. Mol Nutr Food Res. 2014;58(9):1861-72. PubMed PMID: 24974955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen receptor beta is involved in skeletal muscle hypertrophy induced by the phytoecdysteroid ecdysterone. AU - Parr,Maria Kristina, AU - Zhao,Piwen, AU - Haupt,Oliver, AU - Ngueu,Sandrine Tchoukouegno, AU - Hengevoss,Jonas, AU - Fritzemeier,Karl Heinrich, AU - Piechotta,Marion, AU - Schlörer,Nils, AU - Muhn,Peter, AU - Zheng,Wen-Ya, AU - Xie,Ming-Yong, AU - Diel,Patrick, Y1 - 2014/06/27/ PY - 2013/11/04/received PY - 2014/04/28/revised PY - 2014/04/30/accepted PY - 2014/7/1/entrez PY - 2014/7/1/pubmed PY - 2015/7/30/medline KW - Dietary supplement KW - Ecdysterone KW - Estrogen receptor beta KW - Skeletal muscle KW - Traditional Chinese medicine SP - 1861 EP - 72 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 58 IS - 9 N2 - SCOPE: The phytoectysteroid ecdysterone (Ecdy) was reported to stimulate protein synthesis and enhance physical performance. The aim of this study was to investigate underlying molecular mechanisms particularly the role of ER beta (ERβ). RESULTS: In male rats, Ecdy treatment increased muscle fiber size, serum IGF-1 increased, and corticosteron and 17β-estradiol (E2) decreased. In differentiated C2C12 myoblastoma cells, treatment with Ecdy, dihydrotestosterone, IGF-1 but also E2 results in hypertrophy. Hypertrophy induced by E2 and Ecdy could be antagonized with an antiestrogen but not by an antiandrogen. In HEK293 cells transfected with ER alpha (ERα) or ERβ, Ecdy treatment transactivated a reporter gene. To elucidate the role of ERβ in Ecdy-mediated muscle hypertrophy, C2C12 myotubes were treated with ERα (ALPHA) and ERβ (BETA) selective ligands. Ecdy and BETA treatment but not ALPHA induced hypertrophy. The effect of Ecdy, E2, and BETA could be antagonized by an ERβ-selective antagonist (ANTIBETA). In summary, our results indicate that ERβ is involved in the mediation of the anabolic activity of the Ecdy. CONCLUSION: These findings provide new therapeutic perspectives for the treatment of muscle injuries, sarcopenia, and cachectic disease, but also imply that such a substance could be abused for doping purposes. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/24974955/ DB - PRIME DP - Unbound Medicine ER -
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