TRPV1-HEMT biosensor].
Zhongguo Zhong Yao Za Zhi. 2025 Jul; 50(14):3930-3937.ZZ

Abstract

The quality of traditional Chinese medicine(TCM) is a critical foundation for ensuring the stability of its efficacy, as well as the safety and effectiveness of its clinical use. The identification of critical quality attributes(CQAs) is one of the core components of TCM preparation quality control. This study focuses on Jianwei Xiaoshi Tablets and explores their CQAs related to property and flavor from the perspective of taste receptor proteins. Three taste receptor proteins, T1R2, T1R3, and TRPV1, were selected, and a biosensor based on high-electron-mobility transistor(HEMT) was constructed to detect the interactions between Jianwei Xiaoshi Tablets and taste receptor proteins. Simultaneously, liquid chromatography-mass spectrometry(LC-MS) technology was used to analyze the chemical composition of Jianwei Xiaoshi Tablets. In examining the interaction strength, the results indicated that the interaction between Jianwei Xiaoshi Tablets and TRPV1 protein was the strongest, followed by T1R3, with the interaction with T1R2 being relatively weaker. By combining biosensing technology with LC-MS, 16 chemical components were identified from Jianwei Xiaoshi Tablets, among which six were selected as CQAs for sweetness and seven for pungency. Further validation experiments demonstrated that CQAs such as hesperidin and hesperetin had strong interactions with their corresponding taste receptor proteins. Through the combined use of multiple technological approaches, this study successfully determined the property and flavor-related CQAs of Jianwei Xiaoshi Tablets. It provides novel ideas and approach for the identification of CQAs in TCM preparations and offers comprehensive theoretical support for TCM quality control, contributing to the improvement and development of TCM preparation quality control systems.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Liu DH

School of Pharmacy,Fujian University of Traditional Chinese Medicine Fuzhou 350122,China School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center of Chinese Medicine Production and New Drug Development, Ministry of Education Beijing 102488, China.

Han YY

School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center of Chinese Medicine Production and New Drug Development, Ministry of Education Beijing 102488, China.

Huarun Jiangzhong Pharmaceutical Group Co.,Ltd. Nanchang 330096, China.

Wu ZS

MeSH

Drugs, Chinese HerbalBiosensing TechniquesTRPV Cation ChannelsTabletsReceptors, G-Protein-CoupledQuality ControlTasteHumansMass Spectrometry

Pub Type(s)

English Abstract

Journal Article

Language

chi

PubMed ID

40904079

Citation

Liu, Dong-Hong, et al. "[Identification of Critical Quality Attributes Related to Property and Flavor of Jianwei Xiaoshi Tablets Based On T1R2/T1R3/TRPV1-HEMT Biosensor]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 50, no. 14, 2025, pp. 3930-3937.

Liu DH, Han YY, Wang J, et al. [Identification of critical quality attributes related to property and flavor of Jianwei Xiaoshi Tablets based on T1R2/T1R3/TRPV1-HEMT biosensor]. Zhongguo Zhong Yao Za Zhi. 2025;50(14):3930-3937.

Liu, D. H., Han, Y. Y., Wang, J., Li, H. Y., Guo, X. Y., Feng, H. M., He, H., Xu, S. S., Zhong, Z. J., & Wu, Z. S. (2025). [Identification of critical quality attributes related to property and flavor of Jianwei Xiaoshi Tablets based on T1R2/T1R3/TRPV1-HEMT biosensor]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 50(14), 3930-3937. https://doi.org/10.19540/j.cnki.cjcmm.20250321.304

Liu DH, et al. [Identification of Critical Quality Attributes Related to Property and Flavor of Jianwei Xiaoshi Tablets Based On T1R2/T1R3/TRPV1-HEMT Biosensor]. Zhongguo Zhong Yao Za Zhi. 2025;50(14):3930-3937. PubMed PMID: 40904079.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - [Identification of critical quality attributes related to property and flavor of Jianwei Xiaoshi Tablets based on T1R2/T1R3/TRPV1-HEMT biosensor]. AU - Liu,Dong-Hong, AU - Han,Yan-Yu, AU - Wang,Jing, AU - Li,Hai-Yang, AU - Guo,Xin-Yu, AU - Feng,Hui-Min, AU - He,Han, AU - Xu,Shuo-Shuo, AU - Zhong,Zhi-Jian, AU - Wu,Zhi-Sheng, PY - 2025/9/4/medline PY - 2025/9/4/pubmed PY - 2025/9/4/entrez KW - Jianwei Xiaoshi Tablets KW - biosensor KW - chemical composition KW - critical quality attributes KW - traditional Chinese medicine manufacturing SP - 3930 EP - 3937 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 50 IS - 14 N2 - The quality of traditional Chinese medicine(TCM) is a critical foundation for ensuring the stability of its efficacy, as well as the safety and effectiveness of its clinical use. The identification of critical quality attributes(CQAs) is one of the core components of TCM preparation quality control. This study focuses on Jianwei Xiaoshi Tablets and explores their CQAs related to property and flavor from the perspective of taste receptor proteins. Three taste receptor proteins, T1R2, T1R3, and TRPV1, were selected, and a biosensor based on high-electron-mobility transistor(HEMT) was constructed to detect the interactions between Jianwei Xiaoshi Tablets and taste receptor proteins. Simultaneously, liquid chromatography-mass spectrometry(LC-MS) technology was used to analyze the chemical composition of Jianwei Xiaoshi Tablets. In examining the interaction strength, the results indicated that the interaction between Jianwei Xiaoshi Tablets and TRPV1 protein was the strongest, followed by T1R3, with the interaction with T1R2 being relatively weaker. By combining biosensing technology with LC-MS, 16 chemical components were identified from Jianwei Xiaoshi Tablets, among which six were selected as CQAs for sweetness and seven for pungency. Further validation experiments demonstrated that CQAs such as hesperidin and hesperetin had strong interactions with their corresponding taste receptor proteins. Through the combined use of multiple technological approaches, this study successfully determined the property and flavor-related CQAs of Jianwei Xiaoshi Tablets. It provides novel ideas and approach for the identification of CQAs in TCM preparations and offers comprehensive theoretical support for TCM quality control, contributing to the improvement and development of TCM preparation quality control systems. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/40904079/ DB - PRIME DP - Unbound Medicine ER -

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[Identification of critical quality attributes related to property and flavor of Jianwei Xiaoshi Tablets based on T1R2/T1R3/TRPV1-HEMT biosensor].Zhongguo Zhong Yao Za Zhi. 2025 Jul; 50(14):3930-3937.ZZ